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| 黑米中花青苷色素的测定方法研究 |
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吴三桥1,史隋孝2,丁锐1,李新生1
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1. 陕西理工学院,陕西省资源生物重点实验室,陕西汉中,723000;2. 陕西汉中农业学校,陕西汉中,723000
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| 摘要: |
| 以4种黑米为实验材料,用酸性乙醇作为提取溶剂,以色价值为色素含量的评价依据,研究了时间、温度、提取批次和脱脂对黑米中花青苷色素提取的影响.结果表明:80℃,酸性乙醇,60min重复提取2次,可实现90%以上的色素溶出.在最大吸收峰535 nm处测定提取液的ABS值,计算色价,作为黑米中花青苷色素的测定方法,可用于黑米品质分析. |
| 关键词: 黑米 花青苷色素 测定方法 |
| DOI： |
| 分类号:TQ914.1 |
| 基金项目:陕西省教育厅资助项目 |
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| Study on Determination Method of Anthocyanin of Black Rice |
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| Abstract: |
| The optimum conditions for determination of anthocyanin of black rice were studied by using 4 kinds of black rice. The results indicated that the pigment was more than 90% in the liqid removed black rice by extraction in HCL / 95%EtOH(15/85) at 80℃ for 60 min in twice. The extract could be measured for the pigment content by ABS data at the wavelength of 535 nm. |
| Key words: black rice anthocyanin determination method |
