| 摘要: |
| 用固体发酵工艺制得的纳豆中含有大量溶栓活性成分——纳豆激酶.经过生理盐水浸提,(NH4)2SO4分级沉淀.Sephdex柱层析等步骤,可以得一层析纯的纳豆激酶.冷冻干燥品经SDS-PAGE电泳显示有二组分存在. |
| 关键词: 纳豆激酶 纳豆 分离纯化 |
| DOI： |
| 分类号:Q55 |
| 基金项目: |
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| Purificaton and Characterization of Nattokinase |
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| Abstract: |
| The natto made by solid fermentation contains a large amount of thrombuslytic active component-nattokinase. The enzyme was purified to a chromatographic purity with a series of purification procedures including saline extraction, salt-out with ammonium sulfate sephedex gel chromatography and freeze drying. The purified enzyme contained two stainable subunits on the gel of SDS-PAGE. |
| Key words: Nattokinase, natto, purification |
